diff --git a/testing/mozharness/configs/builds/releng_sub_android_configs/64_x86_debug.py b/testing/mozharness/configs/builds/releng_sub_android_configs/64_x86_debug.py
deleted file mode 100644
index 423bf2bcc44e..000000000000
--- a/testing/mozharness/configs/builds/releng_sub_android_configs/64_x86_debug.py
+++ /dev/null
@@ -1,10 +0,0 @@
-# This Source Code Form is subject to the terms of the Mozilla Public
-# License, v. 2.0. If a copy of the MPL was not distributed with this
-# file, You can obtain one at http://mozilla.org/MPL/2.0/.
-
-config = {
-    "stage_platform": "android-x86-debug",
-    "mozconfig_platform": "android-x86",
-    "mozconfig_variant": "debug",
-    "debug_build": True,
-}
diff --git a/testing/mozharness/mozharness/mozilla/building/buildbase.py b/testing/mozharness/mozharness/mozilla/building/buildbase.py
index d1c104bf640d..8a782c057cda 100755
--- a/testing/mozharness/mozharness/mozilla/building/buildbase.py
+++ b/testing/mozharness/mozharness/mozilla/building/buildbase.py
@@ -271,7 +271,6 @@ class BuildOptionParser:
         "rusttests-debug": path_base + "%s_rusttests_debug.py",
         "x86": path_base + "%s_x86.py",
         "x86-lite": path_base + "%s_x86_lite.py",
-        "x86-debug": path_base + "%s_x86_debug.py",
         "x86-profile-generate": path_base + "%s_x86_profile_generate.py",
         "x86_64": path_base + "%s_x86_64.py",
         "x86_64-lite": path_base + "%s_x86_64_lite.py",